This site is a fork of the original NPS@ server
April 9th, 2023: NPS@ is up and running since 25 years (see NEWS).
The NPS@ Web server
NPS@ stands for Network Protein Sequence Analysis (NPSA altered in NPS@ to refer to its access by the Internet network).
NPS@ is an interactive Web server dedicated to protein sequence analysis available for the biologist community.
NPS@ is the "little web brother" of ANTHEPROT and MPSA software. The guiding philosophy is the same for these two software and for this "webware". We hope they are useful and user-friendly.
NPS@ is one service of the "Pôle Rhône-Alpes de Bio-Informatique" (PRABI).
- December 18th, 2019 : this NPS@ fork opening at https://npsa.lyon.inserm.fr by Christophe Combet.
- February 17th, 2003 : NPS@ version 3 is available. This third version was written by Christophe Combet, Céline Charavay, Christophe Geourjon, Christophe Blanchet and Gilbert Deléage.
- April 2nd, 1999 : NPS@ version 2 is available. This second version was written by Christophe Combet, Christophe Geourjon, Christophe Blanchet and Gilbert Deléage.
- April 9th, 1998 : NPS@ version 1 is available. This first version was written by Gilbert Deléage, Christophe Blanchet, Christophe Combet and Christophe Geourjon.
What kind of analysis can you carry out with NPS@ ?
- Homology search by means of pairwise sequence alignment or position-specific scoring matrix / profile:
on sequence databases such as
European Nucleotide Archive (ENA),
UniProt Knowledge Base (UniProtrKB, Swiss-Prot),
Protein Data Bank (PDB),
AlphaFold Database or
ESM Metagenomic Atlas
- Multiple sequence alignment with
- Protein secondary structure prediction including a consensus prediction of those methods. Available methods are
- Primary structure analysis such as :
helix-turn-helix DNA-binding motifs prediction,
amino-acids composition and
What do you need to use NPS@ ?
- one sequence (see help).
- a sequence file in Pearson/FASTA format (see help).
What are NPS@'s strong points ?
- All methods proposed by NPS@ are piped. The output of one method could be the input of another one. For example, after you've performed a sequence similarity search (e.g. BLAST), you can make a database of full or partial sequences. Thereafter, these sequences could be aligned by multiple sequence alignment programs (e.g. KALIGN, MAFFT, MUSCLE), or you can apply NPS@'s methods on each sequence of the database. And this, with no cut and paste, as data transfer are done on server side and format conversion are ensured when needed.
- You can insert protein secondary structure prediction in multiple sequence alignment.
- You can upload your own sequence file and apply NPS@'s methods on it.
- You can download NPSA's data in protein sequence analysis softwares on your local computer for further analysis, to save them or insert them in an article.
- The NPSA link allows you to apply NPS@'s methods on a sequence. Even more, when the sequence comes from a 3D database (PDB), you have some useful links to retrieve and work with 3D data.
- NPS@ offers links on international databases (e.g. ENA, UniProtKB, Swiss-Prot, PDB).
NPS@'s flow chart.
NPS@'s help index page.
To cite NPS@:
NPS@: Network Protein Sequence Analysis
TIBS 2000 March Vol. 25, No 3 :147-150
Combet C., Blanchet C., Geourjon C. and Deléage G.
We will be glad to receive a reprint of article citing NPS@.
This will be a contribution and an encouragement in developing this server.
The CPU time can't exceed 30 minutes for one computation. For longer job, you can contact us.
View number of jobs performed by NPS@.
Last modification time : Wed Apr 5 17:33:46 2023. Current time : Sun May 28 05:35:01 2023. User : email@example.com.